Method of preserving enzyme solutions



Patented Sept. 1, 1931 UNITED STATES PATENT OFFICE LEO WALLERSTEIN, orNEW roux, N. Y., nssrenon 'ro WALLERSTEIN commn z. mo, or NEW YORK, N.Y., A CORPORATION OF NEW YORK METHOD OF ransnnvnre ENZYME SOLUTIONS RDrawing.

Application filed October 6, 1928. Serial 110. 310,911.

.This invention relates to certain improvehalf of one percent by weightof the soluments in a method or process of preserving tion to bepreserved.

enzyme solutions.

In testimony whereof, I have hereunto Enzyme solutions are now largelyused set my hand.

for industrial purposes, such as in the textile industry,-tanningindustry, etc., and such enzyme solutions are prepared in various ways,either from animal products, as pancreas, or vegetable material, such asmalt solutions, or they maybe prepared from bacterial cultures or fungimoulds.

Such solutions are apt to spoil or deteriorate andcertain preservativesor antiseptic substances have been used for preventingsuch-deterioration, as for instance phenol or a phenol derlvative orderivatlves. For some purposes such phenol or its derivatives areunsuitable, or have to be used to obtain the desired preservative actionin suchquantities as to interfere with the stability of the enzymesolutions.

I have discovered that pine oil is especially well adapted for thepreservation of such enzyme solutions and will give the desired 5preservative efiect without interfering with the action of the enzyme orthe stability of the enzyme solutions. This pine oil is used inquantities depending upon the enzyme solution to be preserved and theconditions prevailing in any articular case. I have found that one-halof one percent of ine oil by weight of the solution with w ich it isused is a proportion which gives satisfactory results.

.35 The pine oil may be added to the enzyme solution either during theprocess of producing the solution or to the solution after it has beenprepared, and'furthermore the pine 'oil can be used alone or inconjunction with 40 other preservatives which have no deleterious eflectupon the stability of the solution or its enzymatic action, such forinstance, as form' aldehyde. While the proportion of pine oil stated isa satisfactory one, it will be un- -derstood that this can be varied asdesired to suit any particular solution.

' What I claim:

The method vof preserving enzyme solu- LEO WALLERSTEIN.

